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Chem Impex International
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Oxford Nanopore
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RevoluGen Ltd
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Fisher Molecular Biology
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Promega
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Promega
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Fisher Molecular Biology
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Fisher Molecular Biology
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RevoluGen Ltd
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Molecular Research Center inc
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Promega
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Image Search Results
Journal: Scientific Reports
Article Title: Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery
doi: 10.1038/s41598-024-78066-2
Figure Lengend Snippet: Influence of commercial DNA extraction kits; ZymoBIOMICS™ DNA Miniprep Kit (ZM, Blue), Nanobind CBB Big DNA Kit (NB, orange), Fire Monkey High Molecular Weight (HMW) DNA Extraction Kit (FM, grey) and MagNaPure 96 system (RO, yellow) on DNA concentration (ng µL –1 ) ( a ) and DNA purity in ratio of A260/280 ( b ) and A260/230 ( c ) of eight pathogenic bacteria. Hatched green lines in ( b ) and ( c ) indicate recommended intervals. The values show the mean ± S.E. of three independent replicates and different letters indicate significantly difference between treatments according Duncan’s multiple range test ( p <0.05).
Article Snippet: In this work, we initiated our investigation by an evaluation of three commercial DNA extraction kits: (1) ZM: ZymoBIOMICS™ DNA Miniprep Kit (D4300, Zymo Research, USA), (2) NB: Nanobind CBB Big DNA Kit (Circulomics, USA), (3) FM:
Techniques: DNA Extraction, Molecular Weight, Concentration Assay, Bacteria
Journal: Scientific Reports
Article Title: Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery
doi: 10.1038/s41598-024-78066-2
Figure Lengend Snippet: Genome assembly statistics for all sequencing reads of eight pathogenic bacteria extracted by four different benchmark DNA extraction kits; ZymoBIOMICS™ DNA miniprep kit (ZM), nanobind CBB Big DNA Kit (NB), Fire Monkey High Molecular Weight (HMW) DNA extraction kit (FM) and MagNaPure 96 system (RO). The best assembly statistics from three independent replicates are shown. C, chromosome (chromosome contig); and P, plasmid (plasmid contig).
Article Snippet: In this work, we initiated our investigation by an evaluation of three commercial DNA extraction kits: (1) ZM: ZymoBIOMICS™ DNA Miniprep Kit (D4300, Zymo Research, USA), (2) NB: Nanobind CBB Big DNA Kit (Circulomics, USA), (3) FM:
Techniques: Sequencing, Bacteria, DNA Extraction, Molecular Weight, Plasmid Preparation
Journal: Scientific Reports
Article Title: Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery
doi: 10.1038/s41598-024-78066-2
Figure Lengend Snippet: Influence of genome coverage on raw read length, number of contigs, percentage of completeness and contig N50 of Enterococcus faecium (Efa, a), Streptococcus agalactiae (Sag, b), Streptococcus suis (Ssu, c), and Staphylococcus aureus (Sau, d) extracted by ZymoBIOMICS™ DNA Miniprep Kit (ZM, blue), Nanobind CBB Big DNA Kit (NB, orange), Fire Monkey High Molecular Weight (HMW) DNA Extraction Kit (FM, grey) and MagNaPure 96 system (RO, yellow). Panels without any dots represent exclusions due to lack of coverage to subsample at this depth.
Article Snippet: In this work, we initiated our investigation by an evaluation of three commercial DNA extraction kits: (1) ZM: ZymoBIOMICS™ DNA Miniprep Kit (D4300, Zymo Research, USA), (2) NB: Nanobind CBB Big DNA Kit (Circulomics, USA), (3) FM:
Techniques: Molecular Weight, DNA Extraction
Journal: Scientific Reports
Article Title: Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery
doi: 10.1038/s41598-024-78066-2
Figure Lengend Snippet: Influence of genome coverage on raw read length, number of contigs, contig N50 , and percentage of completeness of Acinetobacter baumannii (Aba, a), Klebsiella pneumoniae (Kpn, b), Pseudomonas aeruginosa (Psu, c), and Salmonella sp. group D (Sgd, d) extracted by ZymoBIOMICS™ DNA Miniprep Kit (ZM, blue), Nanobind CBB Big DNA Kit (NB, orange), Fire Monkey High Molecular Weight (HMW) DNA Extraction Kit (FM, grey) and MagNaPure 96 system (RO, yellow).
Article Snippet: In this work, we initiated our investigation by an evaluation of three commercial DNA extraction kits: (1) ZM: ZymoBIOMICS™ DNA Miniprep Kit (D4300, Zymo Research, USA), (2) NB: Nanobind CBB Big DNA Kit (Circulomics, USA), (3) FM:
Techniques: Molecular Weight, DNA Extraction
Journal: Scientific Reports
Article Title: Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery
doi: 10.1038/s41598-024-78066-2
Figure Lengend Snippet: Influence of genome coverages on percentage of plasmid coverage and circularization (N, non-circular plasmid; Y, circular plasmid) of Enterococcus faecium (Efa, a), Staphylococcus aureus (Sau, b), Acinetobacter baumannii (Aba, c), Klebsiella pneumoniae (Kpn, d), and Salmonella sp. group D (Sgd, e) extracted by ZymoBIOMICS™ DNA Miniprep Kit (ZM, blue), Nanobind CBB Big DNA Kit (NB, orange), Fire Monkey High Molecular Weight (HMW) DNA Extraction Kit (FM, grey) and MagNaPure 96 system (RO, yellow). The percentage of plasmid coverage was calculated by comparing the length of the recovered plasmid to that of the hybrid-reference plasmid.
Article Snippet: In this work, we initiated our investigation by an evaluation of three commercial DNA extraction kits: (1) ZM: ZymoBIOMICS™ DNA Miniprep Kit (D4300, Zymo Research, USA), (2) NB: Nanobind CBB Big DNA Kit (Circulomics, USA), (3) FM:
Techniques: Plasmid Preparation, Molecular Weight, DNA Extraction
Journal: Scientific Reports
Article Title: Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery
doi: 10.1038/s41598-024-78066-2
Figure Lengend Snippet: Summary of DNA isolation kit features used in this study.
Article Snippet: In this work, we initiated our investigation by an evaluation of three commercial DNA extraction kits: (1) ZM: ZymoBIOMICS™ DNA Miniprep Kit (D4300, Zymo Research, USA), (2) NB: Nanobind CBB Big DNA Kit (Circulomics, USA), (3) FM:
Techniques: DNA Extraction, Extraction
Journal: BMJ Open Gastroenterology
Article Title: PCR detection of segmented filamentous bacteria in the terminal ileum of patients with ulcerative colitis
doi: 10.1136/bmjgast-2017-000172
Figure Lengend Snippet: Representative results showing PCR amplification of DNA from stools using PCR primers amplifying (A) SFB sequences and expected to generate an SFB 139 bp specific product or (B) universal PCR primers amplifying bacterial 16S sequences without species specificity. The generated products were analysed by electrophoresis in 2.5% agarose. Expected PCR products are arrowed. Sequences of the employed PCR primers are reported in . M, molecular wt markers; SFB, segmented filamentous bacteria.
Article Snippet: DNA extraction from frozen stool was performed using
Techniques: Amplification, Generated, Electrophoresis, Bacteria